UPF1 RNA Immunoprecipitation from Mini-μ Construct–expressing Cells
نویسندگان
چکیده
منابع مشابه
Rapid method of total RNA mini-preparation from eucaryotic cells.
There have been described a wide variety of methods for the preparation of total RNA from eucaryotic cells (1, 2). These methods are mainly designed for the isolation of RNA from relatively large amounts of cells. In a situation of screening a large number of cell clones, minimized cell cultivation effort and preparation time for total RNA isolation will be of great advantage. We developed a me...
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Chromatin immunoprecipitation (ChIP) is a powerful assay used to probe DNA-protein interactions. Traditional methods of implementing this assay are lengthy, cumbersome and require a large number of cells, making it difficult to study rare cell types such as certain cancer and stem cells. We have designed a microfluidic device to perform sensitive ChIP analysis on low cell numbers in a rapid, au...
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The functional and structural complexity of the myriad of cells in metazoan organisms arises from a small number of stem cells. Stem cells are characterized by two fundamental properties: self-renewal and multipotency that allows a stem cell to differentiate into virtually any cell type. The progression stem cell to differentiated cell is characterized by loss of multipotency, structural and mo...
متن کاملRNA immunoprecipitation for determining RNA-protein associations in vivo.
Similar to chromatin immunoprecipitation (ChIP), RNA immunoprecipitation (RIP) can be used to detect the association of individual proteins with specific nucleic acid regions, in this case on RNA. Live cells are treated with formaldehyde to generate protein-RNA cross-links between molecules that are in close proximity in vivo. RNA sequences that cross-link with a given protein are isolated by i...
متن کاملHuman UPF1 participates in small RNA-induced mRNA downregulation.
MicroRNAs (miRNAs) are endogenous antisense regulators that trigger endonucleolytic mRNA cleavage, translational repression, and/or mRNA decay. miRNA-mediated gene regulation is important for numerous biological pathways, yet the underlying mechanisms are still under rigorous investigation. Here we identify human UPF1 (hUPF1) as a protein that contributes to RNA silencing. When hUPF1 is knocked...
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ژورنال
عنوان ژورنال: BIO-PROTOCOL
سال: 2014
ISSN: 2331-8325
DOI: 10.21769/bioprotoc.1086